miR-21 inhibits apoptosis of Schwann cells following peripheral nerve injury

AIM: To explore the relationship and molecular mechanism between microRNA-21(miR-21) and Schwann cells (SC) following peripheral nerve injury. METHODS: The mRNA expression of miR-21 and phosphatase and tensin homologue deleted on chromosome ten (PTEN) in animal model were detected by real-time PCR. The over-expression of miR-21 and inhibition of miR-21 expression in the Schwann cells according to transfection of lentiviral vectors were performed, the nonspecific miRNA was used as a negative control (NC). The cell apoptosis was measured by flow cytometry. The mRNA expression of miR-21 and PTEN in the cells was detected by real-time PCR. The protein expression of PTEN and cleaved caspase-3 was determined by Western blot. RESULTS: The level of miR-21 was significantly higher and the mRNA level of PTEN was significantly lower in the model of nerve injury than those in control group. miR-21 over-expression decreased the number of apoptotic Schwann cells compared with NC-SC. The mRNA expression of PTEN was down-regulated by over-expression of miR-21. The protein expression of PTEN and cleaved caspase-3 was down-regulated by over-expression of miR-21(P<0.05). CONCLUSION: miR-21 may play an important role in the peripheral nerve injury through inhibiting apoptosis of Schwann cells by down-regulating the expression of PTEN.     

Role of calreticulin-induced mitochondrial damage in high glucose-induced apoptosis of myocardial cells

AIM: To observe the effect of high glucose on the protein expression of calreticulin (CRT) and its association with cell apoptosis and mitochondrial dysfunction in the cardiomyocytes. METHODS: AC-16 cardiomyocytes were randomly divided into normal glucose group, high glucose group, high glucose+ CRT siRNA group and isotonic control group. The cell apoptotic rate, reactive oxygen species (ROS), mitochondrial membrane potential level, respiratory enzyme activity, and protein expression of CRT were observed. RESULTS: Compared with the cardiomyocytes in normal glucose group, the apoptotic rate and ROS production of cardiomyocytes increased in high glucose group, accompanying with the decreases in the mitochondrial membrane potential level and enzyme activitiy of the respiratory chain. The protein expression of CRT was significantly increased in high glucose group. However, compared with high glucose group, high glucose+ CRT siRNA decreased the expression of CRT and attenuated the damage of mitochondria, but CRT siRNA did not reduce the ROS level in cardiomyocytes. CONCLUSION: High glucose brings about CRT over-expression to induce mitochondrial injury, thus increasing myocardial apoptosis.     

二甲苯对小鼠肺组织损伤的初步研究

为了研究二甲苯对小鼠肺组织的损伤情况,以健康成年小鼠作为试验动物,分3个试验组(低、中、高剂量组)1个对照组,给试验组腹腔注射不同剂量二甲苯(0.125、0.25、0.5 mL/kg),给对照组腹腔注射生理盐水.15 d后剖检取材,采用石蜡切片和HE染色技术研究二甲苯染毒后小鼠肺组织结构的变化特点;采用分光光度法测定小...     

Injury Induces a Change in the Functional Characteristics of Cells Recovered from Equine Tendon

Injury initiates a repair process characterized by influx of fibroblasts and the rapid formation of fibrous scar tissue and subsequent tissue contraction. The response to injury and behavior of the different tendon fibroblast populations, however, has been poorly characterized. We hypothesized that the fibroblasts recovered from tendon with acute injury would exhibit different cell properties relating to adhesion, migration and tensegrity. To test this hypothesis we evaluated the ability of fibroblasts recovered from normal and injured equine superficial digital flexor tendons (SDFTs). The injured tendon-derived cells showed greater contraction of the collagen gel but poorer adhesion to pepsin-digested collagen, and migration over extracellular matrix proteins compared to normal SDFT-derived fibroblasts. Thus, the cells present within the tendon after injury display different behavior related to wound healing.     

低温冻害对蚕豆幼苗生理生化特性的影响及RAP-PCR指纹分析

0℃冻害分别处理蚕豆幼苗0、3、6、12 h,测定了蚕豆幼苗叶片的蛋白质、脯氨酸、还原性谷胱甘肽和丙二醛等生理指标,进行了蚕豆低温胁迫下的RAP-PCR指纹分析和抵抗低温冻害的特异基因序列片段的克隆.结果表明:随着低温胁迫的加强,蚕豆幼苗叶片内蛋白质含量呈现先升后降的趋势,脯氨酸相对百分含量逐渐增加,还原性谷胱甘肽含量...     

滇东南橡胶树寒害后次期性害虫危害状况调查

调查了2011年1月云南滇东南橡胶树遭受特大寒害后,引发了以橡胶树小蠹虫类为主的次期性害虫的严重发生和危害,共调查橡胶树6 541株,其中受小蠹虫危害株418株,橡胶树虫蛀率6%,共采集成虫标本232头,种类分属小蠹科Scolytidae、长小蠹科Platypodidae、锯谷盗科Siovanidae、露甲科Nitidulidae等4科4属5种,暗翅材小蠹(Xyleborus semiopacus)和对粒材小蠹(Xyleborus perforans)为优势种;调查还对由于频繁的自然灾害和长年的营养失衡导致橡胶树群体衰退是橡胶林次期性害虫大发生的潜在和根本的原因进行探讨;提出需要对滇东南植胶环境重新研究和评价,对植胶半个多世纪所依循的技术体系和经营体制进行重新审视,以期实现重大变革,使滇东南植胶业得以持续发展。     

热处理降低哈密瓜果实活性氧代谢减轻冷害

为探讨热处理能否激发哈密瓜果实抗冷性,减轻冷害。该文以"西州密25号"哈密瓜为原料,在已有研究的基础上,将哈密瓜在55℃热水中浸泡3 min,以室温(22±2)℃清水浸泡3 min为对照,待其表面水分完全晾干以后,放置于3~5℃机械冷库中贮藏,测定贮藏期间哈密瓜品质及生理指标。结果表明,与对照相比,热处理诱导哈密瓜果肉过氧化氢H_2O_2和超氧阴离子O_2~-含量短暂增加,但明显减少贮藏中后期(14~35 d)H_2O_2,O_2~-的积累(P0.05),提高活性氧清除酶过氧化物酶和超氧化物歧化酶的活性(P0.05),抑制细胞膜相对渗透率和丙二醛含量上升,降低贮藏后期的冷害发生率(P0.05),缓解果实可溶性固形物和抗坏血酸含量下降(P0.05),保持果实较好的品质。热处理主要通过诱导活性氧信号分子,提高活性氧清除酶活性、减少膜脂过氧化作用,从而减轻果实的冷害。研究结果为哈密瓜采后贮藏技术提供理论参考。     

农业环境保护中的酸雨监测技术

酸雨伤害植物的监测技术包括对植物叶片表观伤害监测与生理伤害监测,后者选择的参数主要有植物光合速率、气孔导度、细胞质膜透性、叶绿素含量消长、细胞pH变化、活性氧防御系统的应激反应等。本文在介绍测定方法同时,比较分析了各种方法的优点与不足,探讨了酸雨监测技术在农业环境监测、环境评价、酸沉降区域农业区划与环境保护中的作用,指出今后研究应重视完善监测体系和拓展监测对象。     

Impact of defoliation on yield of group IV soybeans in Mississippi

Field experiments were conducted during 2009 and 2010 to evaluate the effects of defoliation on maturity group IV soybeans, Glycine max (L.) Merr., grown in Mississippi. During each year, two locations were planted with maturity group IV soybeans that were subjected to various levels of defoliation during R3, R5, and R6 growth stages. Soybeans were subjected to various levels of defoliation within the upper 50% of the plant canopy, lower 50% of the plant canopy, and whole-plant canopy. There was greater yield loss from defoliation occurring in the upper plant canopy compared with the lower plant canopy during R3 and R5 stages, but no difference between canopy regions during R6 stage. Yield loss from whole plant defoliation was greater than upper or lower canopy defoliation. Results confirmed that soybeans during R3 and R5 stages are more susceptible to yield loss than during R6. However, yield losses were not significantly different between R 3 and R5 until defoliation exceeded 63%. Dynamic economic injury levels were determined for each growth stage based on yield loss equations, value of the crop, and cost of control and can be used as a basis for developing action thresholds in high-yielding soybean production environments.